IVD EIT™
In Vitro Depth of Injury Eye Irritation Test
The only nonanimal test that classifies all four GHS eye hazard categories: NC, 2B, 2A, and 1. Over 90% accuracy. Uses discarded food-source tissue.
Category 1
Serious eye damage
Category 2A
Serious eye irritation
Category 2B
Mild eye irritation
Not Classified
No significant risk
Only NAM covering all 4 GHS categories
4 of 4
GHS categories covered
>90%
Overall accuracy
Broad
Application domain
Food-source
Eye material origin
How IVD EIT™ works
The IVD EIT™ is the only nonanimal test method capable of discriminating all four GHS eye hazard categories.
The method is based on the principle that the depth of injury in the corneal tissue is the primary determinant of GHS eye hazard category. Superficial stromal injury corresponds to Category 2B or 2A; deeper injury extending into the deep stroma corresponds to Category 1. No stromal injury corresponds to Not Classified.
Tissues are processed into OCT-embedded tissue sections and stained for viability (Viability Stain Kit) or DNA fragmentation (TUNEL Kit). The depth of fluorescent staining in the corneal tissue directly predicts the GHS hazard category with greater than 90% accuracy.
IVD EIT is particularly valuable for pharmaceutical and excipient testing, where the full GHS classification spectrum is required and where the mechanistic relevance of a corneal tissue model is important.
Step-by-step protocol overview
Obtain Tissue
Fresh food-source tissue from certified supplier. Use within 24 hours of collection.
Corneal exposure
Overlay with tear, apply 100 µL of test material using dosing rings and exposure stabilizers from the Main Kit. Rinse with tear, incubate for 18 hours, fix tissue.
OCT processing
Embed the cornea in OCT compound and cryosection.
Stain sections
Apply Viability Stain Kit (IVDV3648) or TUNEL Kit (T3212).
Fluorescent imaging
Image sections using a fluorescent microscope.
Classify
Measure depth of injury and assign GHS category: NC, 2B, 2A, or 1.
Application domains
Pharmaceuticals
Ophthalmic drugs, excipients, topical formulations requiring full GHS classification
Industrial Chemicals
Chemicals requiring full GHS eye hazard classification for SDS and labeling
Cosmetics
Products where GHS 2A/2B distinction is needed beyond NC determination
Agrochemicals
Pesticides and formulations requiring complete GHS eye hazard profiling
Which kit do I need?
Start with the Main Kit
IVD EIT Main Kit (IVDM3544) is required for every run. Contains all reagents for corneal exposure and processing.
Choose your viability stain
Pair with Viability Stain Kit (IVDV3648) for live/dead staining, or TUNEL Kit (T3212) for DNA fragmentation detection.
Equipment check
A fluorescent microscope, OCT embedding equipment, and a cryostat or microtome are required.
IVD EIT™ Kits
Each kit row includes a Full kit details button. Click to open tabbed support content with protocol steps, materials list, and downloadable documents.
IVD EIT™ Main Kit
SKU IVDM3544
Performs the In Vitro Depth of Injury Eye Irritation Test. Classifies all 4 GHS eye hazard categories. Requires Viability Stain Kit or TUNEL Kit, sold separately.
Requires IVD Viability Stain Kit (IVDV3648) or TUNEL Kit (T3212), sold separately. Fluorescent microscope required.
$1,200
per kit
Ships within 3–5 business days
IVD EIT™ Viability Stain Kit
SKU IVDV3648
Rapid, easy-to-use live/dead fluorescent viability stain for OCT-processed corneal tissue sections. Results in approximately 1 hour.
Stains 36 sections. Requires fluorescent microscope (not included). Use with IVD EIT Main Kit.
$600
per kit (36 sections)
Ships within 3–5 business days
TUNEL Stain Kit
SKU T3212
Detects DNA fragmentation in paraffin or OCT-processed tissue sections. Optimized two-step stain and counterstain with very high signal-to-noise ratio.
Stains and counterstains 36 sections. Requires fluorescent microscope (not included). Alternative to Viability Stain Kit.
$600
per kit (36 sections)
Ships within 3–5 business days
Free shipping on orders over $500 · Volume discounts available · Contact us
Need contract testing?
LebrunLabs.co offers contract testing using IVD EIT™ and other InChemico methods.